Cytxon
Cytxon
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    • Home
    • About
      • About Company
      • Vision
      • Mission
      • People
    • Programme
      • Academic Internship
      • Paid Internship
    • Services
    • Research & Developement
      • Products
      • Publications
      • Patents
    • Gallery
    • Contact Us
  • Home
  • About
    • About Company
    • Vision
    • Mission
    • People
  • Programme
    • Academic Internship
    • Paid Internship
  • Services
  • Research & Developement
    • Products
    • Publications
    • Patents
  • Gallery
  • Contact Us

Our services

  1. Cell Culture Based Assay
  2. Stem Cell Based Assay 
  3. Microbiology
  4. Flow cytometry Based Assay
  5. Fluorescence Microscopy based studies  
  6. In vivo drug testing 
  7. In vitro drug testing 
  8. Phytochemistry 
  9. Molecular Biology Based Assay 
  10. Proteomics 
  11. Analytical 
  12. Bioinformatics  
  13. Nanotechnology Based Services  
  14. Profile Based Assay 
    1. Anti –cancer activity   
    2. Anti-Diabetic activity  
    3. Anti-Inflammatory activity  
    4. Hepatoprotective Activity  
    5. Cardio-protective activity  
    6. Neuroprotective Activity 
  15. METASTASIS AND ANGIOGENESIS ASSAY
  16. Functional Cell Stress Assays
  17. Special pre clinical tests
  18. Safety testing program of pharmaceuticals medical devices, chemicals, food  additives 
  19. Training Programs

Cell Culture Based Assay

  • In vitro Screening of Drug Toxicity, 
  • Mode of Action, 
  • Cytotoxicity Assay:- SRB Assay ,LDH Assay, MTT Assay, Clonogenic Assay   
  • Angiogenesis Assay 
  • Cell Migration Assay 
  • Scratch Assay 
  • Cytoprotective Assay 
  • Skin Irritation Assay 
  • Absorption Assay 
  • Eye irritation 
  • Skin corrosion 
  • Skin sensitization 
  • Transdermal Permeability Testing 
  • Hepatotoxicity 
  • Cytotoxicity in fibroblast cell lines (MTT and Neutral red) 
  • Phospholipidosis and Steatosis Assay 
  • Lysosomotropism 
  • Hemolysis assay 
  • Phototoxicity Testing 
  • Immunogenicity studies 
  • Mitochondrial Toxicity 
  • hERG Cardiotoxicity  
  • Phospholipidosis 

Stem Cell Based Assay

  • Isolation and maintenance of Adult Stem cells and umbilical cord blood stem cells 
  • Characterization of MSCs/AMSCs/HSCs/NSCs 
  • Neuronal differentiation studies 
  • Osteogenic differentiation studies 
  • Adipogenic differentiation studies  
  • Dendritic and other immune cell generation from HSCs/MSCs 
  • Signalling pathways governing stem cells:  Notch, Wnt, Hedgehog, etc. 
  • CFU Formation test 

Microbiology

  • Isolation and culturing of Bacteria/Fungi/Algae. 
  • Biochemical Characterization and identification of Bacteria/Fungi/Algae 
  • Identification and characterization of microbes by16S and 18S rRNA sequencing 
  • Minimum Inhibition Concentration 
  • Antibacterial/antifungal Activities 
  • Isolation of Industrially and Clinically important Microbes and Characterization. 
  • Pre-Biotic Assay 
  • Pro-Biotic Assay  
  • Fermentation Technology 
  • Enzyme Production/Enzyme isolation/Enzyme characterization 
  • Gelatin/Casein Zymography 
  • Sterility/contamination Testing 
  • Anti-Quorum Sensing Activity 
  • Isolation and Characterization of microbes in clinically diseased patients 
  • Production of Bioplastic using bacteria 

Flow cytometry Based Assay

  

  • Detection of Stem Cells Markers and Differentiation Markers 
  • Cell Cycle Analysis (Propidium Iodide staining)
  • DNA Fragmentation Studies (TUNEL Assay)
  • Cell Specific Marker Studies 
  • Plant Ploidy and Genome Size Analysis
  • Sperm Analysis (Mitochondrial depolarization assay/TUNEL Assay)
  • Immunology (Cytokinin/Interleukins/TNF-α/CD-4/CD-8/)
  • Hematology (Immunophenotyping/ erythrocyte analysis/ leukocyte analysis/ platelet analysis/ Quantification of antigens or antibodies).
  • Pre-natal diagnosis
  • Pathology (etiological diagnosis)
  • Microbiology (Quantification of antigens/TUNNEL assay/Cell viability/food safety/Bacterial Transformation/Bacterial membrane potential assay)
  • Genetics (Genome content/Ploidy analysis/detection of functional genes).
  • Toxicity Testing (Cell Toxicity/viability assays)
  • Anti Cancer Studies (Cancer Cell Toxicity/viability assays/ Apoptosis /necrosis assay/Mitochondrial membrane potential (Δψ)/ DNA Damage assays/ cell cycle analysis/ Cancer marker specific analysis).

Fluorescence Microscopy based studies

  

  • Immunocytochemistry (ICC) based marker analysis
  • Immunohistochemistry (IHC) based marker studies 
  • Karyotyping
  • Apoptosis studies by Nuclei and cytoplasmic staining 

In vivo drug testing

  • Anticancer Activity
  • Hepatoprotective Activity
  • Wound Healing Activity
  • Anti- Diabetic Activity
  • Anti –Convulsent Activity
  • Neuroprotective Activity
  • Anti –Venom Activity
  • Anti –Arthritic Activity 
  • Anti –Oxidant Activity
  • Anti-Tumor Activity
  • Acute Toxicity Studies
  • Anti-Inflammatory Activity
  • Anti-Depressant Activity
  • Behavioral Pharmacological Studies
  • Infertility Studies

In vitro drug testing

  • In vitro anti-fungal activity
  • In vitro anti-bacterial activity
  • In vitro anti-viral activity
  • Minimum Inhibition Concentration (MIC) determination
  • In vitro anthelmintic activity
  • In vitro larvicidal activity
  • Cytotoxicity studies 
  • Cell viability studies by trypan blue exclusion assay
  • In vitro anti-arthritic activity
  • In vitro anti-diabetic activity
  • In vitro anti-oxidant assays
  • In vitro anti-quorum sensing
  • In vitro hepatoprotective activity
  • In vitro neuroprotective activity
  • In vitro cardioprotective activity
  • In vitro anti-inflammatory activity

Phytochemistry

  • Phyto-extraction
  • Isolation and Characterization of phytochemicals 
  • Spectral analysis and identification of phytochemicals
  • Biological Activity of plant extracts/phytochemicals 

Molecular Biology Based Assay

  • PCR
  • ELISA
  • SDS-PAGE
  • AGE
  • Western Blotting, Southern Blotting, Northern Blotting
  • FISH
  • Construction of c-DNA Library 
  • Protein/DNA/RNA Sequencing
  • Transformation
  • DNA Binding, DNA Cleavage and DNA Fragmentation assays. 
  • RAPD, RFLP
  • DNA fingerprinting
  • Bacterial Cloning and transformation 

Proteomics

  • Protein Expression Profiling By 2D-Gel Electrophoresis
  • 2D Gel-Image Analysis
  • MALDI-TOF and Data Analysis
  • Protein Function analysis
  • 2D Gel Electrophoresis Data Analysis 
  • Pathways Analysis and Interpretation

Analytical


  • HPLC
  • PREP-HPLC
  • FLASH-HPLC
  • GC-MS
  • LC-MS
  • FTIR
  • 1H-NMR and 13C- NMR
  • XRD
  • SEM
  • TEM
  • Chromatography:- TLC, Paper, Column, Affinity, Ion Exchange

Bioinformatics

  •  In-silico Drug Designing
  • Quantitative Structure Activity Relationship (QSAR)
  • Molecular Docking
  • Sequence Analysis
  • DNA, RNA and Protein Sequence Analysis.
  • Homology Modeling
  • Phylogenetic Analysis
  • Primer Designing
  • Recombination Detection
  • Protein/Gene Function annotation
  • Database Development 

Nanotechnology Based Services

 

  • Synthesis Of  Nano-Materials 
  • Characterization Of Nano-Materials
  • Mass Production
  • Functionalization of Nanoparticles
  • Biological Activity of Nanoparticles

PROFILE BASED ASSAY

Anti–cancer activity

Anti-Inflammatory activity

Anti-Diabetic activity


  1. Primary screening of the drug using cytotoxicity assays :- LDH,MTT, XTT, MTS,SRB
  2. Apoptosis /necrosis assay:- annexin-v , Mitochondrial membrane potential (Δψ)
  3. Apoptosis:- caspase-3, Extrinsic pathway- caspase-8, Extrinsic pathway-caspase-9, bax/bcl-2,
  4. DNA Dammage- tunel assay,comet assay
  5. Cell cycle analysis
  6. Cancer marker specific analysis- Phospho-ERK assay, C-Jun test, Ca2+ assay, HIF-1α assay, NF-kappa B/p65, cAMP assay, Phospho-CREB assay, STAT 1,2,3 & 6 assays, EGFR assay, Phospo-mTOR assay, Akt assay, Phosphor-Akt (p-Akt) assay, p38/p53 assay. 

Anti-Diabetic activity

Anti-Inflammatory activity

Anti-Diabetic activity

 

  1. Primary screening of the drug using cyto-toxicity assays :- LDH, MTT,  XTT, MTS, SRB
  2. Biochemical tests:- α-glucosidase inhibition assay,
  3. In vitro cell line based assay: - Glucose uptake assay.
  4. Marker based assays:- Insulin receptor Binding studies,GSK-3β assay.

Anti-Inflammatory activity

Anti-Inflammatory activity

Anti-Inflammatory activity

 

  1. Primary screening of the drug using cyto-toxicity assays :- LDH, MTT,  XTT, MTS, SRB
  2. Cytokine and chemokine assay 
  3. COX activity assay 
  4. TNF-α,
  5. Interlukin expression

Hepatoprotective Activity

Cardio-protective activity

Anti-Inflammatory activity


  1. Primary screening of the drug using cyto-toxicity assays :- LDH, MTT,  XTT, MTS, SRB
  2. Proliferative ,protective assay with appropriate concentration against suitable toxin :-
  3. Pre-treatment/Post-treatment/Co-treatment experiments
  4. Serum based Liver function tests:- AST, ALP, ALT, Total Bilirubin, Total Protein, Lipid profile.
  5. Liver tissue based biochemical tests: CAT, GPx, GST, LPO, SOD.
  6. Marker expression analysis

Cardio-protective activity

Cardio-protective activity

Cardio-protective activity


  1. Primary screening of the drug using cyto-toxicity assays :- LDH, MTT,  XTT, MTS, SRB
  2. Proliferative ,protective assay with appropriate concentration against suitable toxin 
  3. Pre-treatment/Post-treatment/Co-treatment experiments
  4. Biochemical Assay:- Troponin-T,LDH, TNF-α, Antioxidant status
  5. Marker expression analysis

Neuroprotective Activity

Cardio-protective activity

Cardio-protective activity


  1. Primary screening of the drug using cyto-toxicity assays :- LDH, MTT,  XTT, MTS, SRB
  2. Proliferative ,protective assay with appropriate concentration against suitable toxin :-
  3. Pre-treatment/Post-treatment/Co-treatment experiments
  4. Biochemical Assay- Gpx, Gox
  5. Marker expression analysis 

METASTASIS AND ANGIOGENESIS ASSAY

  • Cell migration assays
  • Scratch assay and Tube formation assay 
  • Angiogenesis signaling pathways: (H1F1α, SMAD, p38, E-cadherin, PI3K) 

Functional Cell Stress Assays

  • Biochemical and Cell line based assays
  • NADPH Reducing assay
  • ROS formation assay 
  • iNOS formation assay 
  • Lipid peroxidation assay 
  • Glutathione depletion assay
  • Mitochondrial membrane potential assays 
  • Hypoxia signaling marker analysis
  • Anti-oxidant assays: SOD1, SOD2, catalase (CAT), GSH, GPX and GSR

Special pre clinical tests

Safety testing program of pharmaceuticals, medical devices, chemicals, food additives

  • In vitro Mammalian Chromosome Aberration Test in Cultured Human Peripheral Blood Lymphocytes/CHO cell line - OECD 473.
  • In vitro Mammalian Cell Gene Mutation Test (using mouse lymphoma L5178Y tk +/- cells) - OECD 476.
  • In vitro Micronucleus Test - OECD 487.
  • Biocompatibility studies on medical devices
  • Skin irritation studies


Absorption studies: 

  • Cell permeability assays (Caco2 and MDCK cells)


Drug – Drug Interaction studies: 

  • Cytochrome P450 Inhibition
  • Phototoxicity and Photos
  • ensitisation Screening 

CONTRACT BASED Industrial research application

MONOCLONAL/ POLYCLONAL ANTIBODY PRODUCTION

ENZYME PRODUCTION SERVICE

Training Programs

Precision Agriculture

Our precision agriculture solutions use advanced analytics and data-driven insights to optimize crop yields, minimize waste, and promote sustainable farming practises.

Environmental Biotech

Our environmental biotech solutions are focused on developing innovative technologies for waste management, pollution control, and environmental remediation.

Industrial Biotech

Our industrial biotech solutions use biologically-derived materials to create sustainable alternatives to traditional manufacturing processes, reducing waste and improving efficiency.

Health and Wellness

Our health and wellness products leverage the latest advances in biotech to promote optimal health and well-being, from dietary supplements to advanced diagnostics.

Biofuel

Our biofuel solutions use renewable biomass to create clean-burning fuels, reducing dependence on fossil fuels and promoting a more sustainable energy future.

Biotech Consulting

Our biotech consulting services provide expert guidance and support to biotech companies and organizations, helping them navigate the complex landscape of biotech innovation.

Cytxon Biosolutions Pvt. Ltd.

Copyright © 2022 Cytxon Biosolutions - All Rights Reserved.

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